Attenuated Total Reflectance (ATR)- a versatile FT-IR sampling tool
FT-IR Spectroscopy is an acknowledged tool for identification and characterization of materials on the basis of presence of functional groups present. The technique has been…
FT-IR Spectroscopy is an acknowledged tool for identification and characterization of materials on the basis of presence of functional groups present. The technique has been…
Colour measurements are the basis of several quantitative estimations and are based on light absorbance by the coloured medium. In addition to absorption several methods…
It is a well-known fact that ordinary white light comprises of a mix of different wavelengths or colours. We are all familiar with the different…
Ideally the absorbance spectrum of a solution containing a single analyte should be a single absorption band at the wavelength of maximum absorbance. However, in…
Absorbance studies have become a base for examining various solutions and particles. Under these, the light gets passed through the solutions in a defined format.…
A clear understanding on the concept of Double beam and Single beam optics is an absolute must for a spectroscopist. A common misconception is that in a double beam instrument two beams pass simultaneously through the sample cell and provide a sensitivity advantage over single beam systems. For that matter the more the number of beams passing through the sample cell better will be the sensitivity. Today’s Lab Byte session will help dispel such misconceptions. In Double beam design one beam  component passes through the sample cell and the other through the blank solvent taken  in the reference cell. This arrangement helps compensate errors arising due to changes in any of the operational parameters such as power line fluctuations, stray light and solvent absorbance during absorbance observations which contributes to higher accuracy and precision of results.