Comments on: What is Chromatography?

By: Dr. Saurabh Arora

Dr. Saurabh Arora — Mon, 09 Mar 2015 05:16:42 +0000

In reply to Mohan Thippeswamy. Thank you Mohan, we are working on some articles on method development and pKa and will be sharing them soon.

By: Mohan Thippeswamy

Mohan Thippeswamy — Thu, 05 Mar 2015 08:41:32 +0000

Dear Sir,

I had learnt a lot from your lab training module. Plz send me the basics of Method Development for any compound/molecule/biomolecule to develop a method. And also plz give me suggestions on regarding pKa of molecule.

Thanks & Regards
Mohan Thippeswamy

By: Dr. Deepak Bhanot

Dr. Deepak Bhanot — Wed, 16 Jul 2014 04:02:27 +0000

In reply to desmo. Hi Desmo, Yes you are right-sample preparation is the key to success of any analysis.As your application is specific it would be best for you to refer to literature or search the references of standard methods for getting best results for your separations.

By: desmo

desmo — Mon, 07 Jul 2014 12:24:47 +0000

Hello, I have the articles have been useful. I have been testing with capillary chromatography and electrophoresis. Basically I am using the device as LC(pressure over time) with added separation factors of the electricity. It seems to me that the sample preparation method has everything to do with accuracy and repeatability of results. My question: how does one create reference standards using the compounds? I have several phenol compounds(8-20) in solution using ethanol as liquid phase. I think I need to separate them as best I can, create two identical solutions and spike one with a reference, then test. Best regards, Desmo.

By: Dr. Deepak Bhanot

Dr. Deepak Bhanot — Sat, 21 Sep 2013 12:45:34 +0000

In reply to Mohammed shahid.

Dear Shahid,
Glad to note that you found the article useful and informative.Chromatographc techniques are useful in analysis of base oils. In general there are two main applications.Firstly for studies on boiling range distribution(Ref ASTN D7500-12) and secondly for determination of Total Polyaromatic Content.A useful link is being provided on a handbook on base oils http://www.engnetglobal.com/documents/pdfcatalog/NYN001_200412073535_Base%20oil%20handbookENG.pdf
Hope you find the response useful for your applications.
Regards

By: Mohammed shahid

Mohammed shahid — Sat, 21 Sep 2013 06:11:16 +0000

Hello Sir
Thanks a lot for ur update and most knowledgeable information regarding HPLC .M working in a petroleum compny I want to know whats application and parameter of HPLC AND GC for base oil SN 150,SN300 PLZ update me.

THANKS
SHAHID

By: Dr. Deepak Bhanot

Dr. Deepak Bhanot — Wed, 18 Sep 2013 09:23:21 +0000

In reply to ASIT KUMAR DE. Dear Asit, I believe that the concept is universal irrespective of the analytical technique.The only difference is that in case of chromatographic analysis it refers to adddition of the organic compound whereas in AAS it refers to addition of the same element in known concentration whose composition matches the sample composition.

By: ASIT KUMAR DE

ASIT KUMAR DE — Mon, 16 Sep 2013 15:41:44 +0000

Dear Sir,
I am woking with a drug laboratory now and I am learning a very good concepts with your lab training programme.But I wok with an AAS in our laboratory.But I don’t understand the standard addition techniques in AAS.
So, if you kindly give the proper concept of standard addition techniques in AAS instrument with an example elaborately I shall be grateful to you.
Thanking You, Your’s faithfully,
Date-16.9.2013 Asit Kumar De