Comments on: Role of Buffers in Reverse Phase HPLC Separations https://lab-training.com/role-of-buffers-in-reverse-phase-hplc-separations/ Experience Beyond Books Mon, 28 Aug 2017 06:17:12 +0000 hourly 1 https://wordpress.org/?v=6.7.2 By: rits https://lab-training.com/role-of-buffers-in-reverse-phase-hplc-separations/#comment-80108 Mon, 28 Aug 2017 06:17:12 +0000 https://lab-training.com/?p=15038#comment-80108 it wasvery useful. Pls continue to write.

]]> By: Dr. Deepak Bhanot https://lab-training.com/role-of-buffers-in-reverse-phase-hplc-separations/#comment-78883 Wed, 12 Jul 2017 10:24:04 +0000 https://lab-training.com/?p=15038#comment-78883 In reply to Adisa MJ.

My advice would be to use some other buffer medium as viscous media will result in shortening the useful life of the column. The buffer media should be free flowing.

]]> By: Adisa MJ https://lab-training.com/role-of-buffers-in-reverse-phase-hplc-separations/#comment-78851 Tue, 11 Jul 2017 06:25:54 +0000 https://lab-training.com/?p=15038#comment-78851 sir,
how can i separate my isolate from buffer (triethanolamine)which is viscous liquid recovered from hplc

]]> By: Dr. Deepak Bhanot https://lab-training.com/role-of-buffers-in-reverse-phase-hplc-separations/#comment-18701 Sat, 28 Jun 2014 03:53:07 +0000 https://lab-training.com/?p=15038#comment-18701 In reply to nafise jarrah.

Dear Nafise,
Macrolides have been determined commonly in different foods with the help of UV-Vis diode array detector simultaneously at different wavelengths.As per your requirements and applications you have to refer to some chromatographic journals to develop your own test conditions.
Regards

]]> By: nafise jarrah https://lab-training.com/role-of-buffers-in-reverse-phase-hplc-separations/#comment-18082 Wed, 25 Jun 2014 09:32:40 +0000 https://lab-training.com/?p=15038#comment-18082 Dear Dr Deepak Bhanot,
I am writing to you in order to ask for some details about your World Support Program.
I need some information about determination of antibiotics (Macrolids) by HPLC UV-detector.
Could I ask you to guide me?
I look forward to hearing from you.
Yours faithfully

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