Comments on: How to Mix HPLC Mobile Phase Solvents for consistency of results? https://lab-training.com/how-to-mix-hplc-mobile-phase-solvents-for-consistency-of-results/ Experience Beyond Books Sun, 22 Nov 2020 13:36:02 +0000 hourly 1 https://wordpress.org/?v=6.7.2 By: Dr. Deepak Bhanot https://lab-training.com/how-to-mix-hplc-mobile-phase-solvents-for-consistency-of-results/#comment-79373 Fri, 04 Aug 2017 04:31:27 +0000 https://lab-training.com/?p=16420#comment-79373 In reply to A. Garcia.

Many thanks Garcia for appreciating our efforts.Online mixing is preferable due to its accuracy over offline mixing. The software on modern systems compensates for volume changes, if any. The temperature changes are not significant enough to affect the chromatography or damage any internal components.

]]> By: A. Garcia https://lab-training.com/how-to-mix-hplc-mobile-phase-solvents-for-consistency-of-results/#comment-79361 Thu, 03 Aug 2017 18:56:16 +0000 https://lab-training.com/?p=16420#comment-79361 Hallo Dr. Bhanot. First of all thank you very much for your useful and interesting posts on HPLC. I have a question regarding the topic of mixing solvents in isocratic methods: what is better, mix them offline or online? I learnt that it is more adecuate offline due to the effects of temperature and volume changes that may badly influence the chromatography and damage the pumps in long term and so on. However, another approach is that pumps mix more accurate than people and thus isocratic HPLC will be performed by mixing solvents in the mixing chamber. Thank you very much in advance!

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