Comments on: Peak Height or Peak Area? – Which is the right choice for quantitative chromatographic calculations

By: Collins

Collins — Sat, 30 May 2020 04:57:31 +0000

LAb-training.com is a great scientific asset indeed.

By: Dr. Saurabh Arora

Dr. Saurabh Arora — Sun, 07 Jul 2019 14:55:28 +0000

In reply to Sharad Raut. Signal to noise is very important for establishing the Limit od Detection and Limit of Quantification for any method. Usually, S/N of 3 is acceptable for LOD and 10 for LOQ.

By: Dr. Saurabh Arora

Dr. Saurabh Arora — Sun, 07 Jul 2019 14:52:45 +0000

In reply to rumaisa. Hi the area by itself is not god enough to decide. You need to look at the signal to noise ratio and repeatability of the response. So a peak area of 1000 will be good if the noise has an area of 100 (S/N - 10) but is noise is more it will not be.

By: rumaisa

rumaisa — Mon, 24 Jun 2019 05:21:00 +0000

Hi Dr.
I was wondering what is the limit of the peak area? if it less than 1000 does the area seems valid?

By: Sharad Raut

Sharad Raut — Tue, 18 Jun 2019 22:04:02 +0000

Why need a signal to noise ratio in HPLC .
And which purpose use this test.

By: Dr. Deepak Bhanot

Dr. Deepak Bhanot — Mon, 04 Dec 2017 04:49:40 +0000

In reply to Tek Bahadur Bohra. Nice to hear from your side. It seems you have put in so many years of service.how are you and where are you located.

By: Tek Bahadur Bohra

Tek Bahadur Bohra — Thu, 30 Nov 2017 06:33:22 +0000

HI, Dear Sir,
I am Tek Bahadur Bohra from SUN Pharma(Erstwhile RANBAXY).
Nice to see in this page how are you sir.

By: Dr. Deepak Bhanot

Dr. Deepak Bhanot — Sat, 16 Sep 2017 05:08:07 +0000

In reply to roshan d patil. Modern software takes care of best fits in calculations and you do not have to bother yourself with graphic details. Manual integrations can introduce errors arising from personal bias.

By: roshan d patil

roshan d patil — Fri, 01 Sep 2017 11:18:15 +0000

hi sir,

I need to know about points that you will look at when reviewing the chromatograms for a batch, for Assay results. Focus on Data Integrity.

What manual integrations are prohibited/not allowed?how it can be detected from empower/chromaleon?

By: Dr. Deepak Bhanot

Dr. Deepak Bhanot — Mon, 26 Dec 2016 09:56:34 +0000

In reply to Rajesh Tripathi. Manual calculation is superseded in today's instruments. The software provides you the area under a peak in terms of digital counts.It also takes into account the best fit and takes care of the underlying parameters so you are saved a lot of calculation and personal judgements.