Comments on: Prevention of Retention Time Drifts in HPLC Analysis https://lab-training.com/prevention-of-retention-time-drifts-in-hplc-analysis/ Experience Beyond Books Wed, 28 Jun 2017 10:42:32 +0000 hourly 1 https://wordpress.org/?v=6.7.2 By: Dr. Saurabh Arora https://lab-training.com/prevention-of-retention-time-drifts-in-hplc-analysis/#comment-78503 Wed, 28 Jun 2017 10:42:32 +0000 https://lab-training.com/?p=15252#comment-78503 In reply to VISHAL AHUJA.

Hi Vishal this might be because your sample diluent and mobile phase are different.

]]> By: VISHAL AHUJA https://lab-training.com/prevention-of-retention-time-drifts-in-hplc-analysis/#comment-76117 Wed, 22 Mar 2017 15:56:28 +0000 https://lab-training.com/?p=15252#comment-76117 Sir i am working on Xylitol production and i have tried HPLC for estimation with RID. When i inject standard in different concentrations peak shifted towards less retention time with lower concentration. What could be the base possible explanation for that?

]]> By: Dr. Deepak Bhanot https://lab-training.com/prevention-of-retention-time-drifts-in-hplc-analysis/#comment-62940 Fri, 19 Feb 2016 11:00:48 +0000 https://lab-training.com/?p=15252#comment-62940 In reply to kamella.

In reply to Kamella
See the response to Katrina provided by Dr Saurabh.Try to replicate your operating conditions for each run and this will surely narrow down the retention time difference.

]]> By: kamella https://lab-training.com/prevention-of-retention-time-drifts-in-hplc-analysis/#comment-62732 Wed, 10 Feb 2016 10:27:52 +0000 https://lab-training.com/?p=15252#comment-62732 thanks sir for your article.
btw, can you teach me about value of drifting time. first the result is 4.3 and next analysis move to 4.4, i mean this moven is significant sir. do yo know rule to certain the peak is equal sir?

]]> By: Dr. Saurabh Arora https://lab-training.com/prevention-of-retention-time-drifts-in-hplc-analysis/#comment-58068 Sat, 20 Jun 2015 06:22:03 +0000 https://lab-training.com/?p=15252#comment-58068 In reply to Kishor.

Hi Kishor, Both these factors can have varied effects on the retention time depending on the polarity and nature of solvents, column, mobile phase and analytes. For example THF as diluent can reduce RT for non polars and increase it for polars.

]]> By: Kishor https://lab-training.com/prevention-of-retention-time-drifts-in-hplc-analysis/#comment-57537 Thu, 04 Jun 2015 10:31:19 +0000 https://lab-training.com/?p=15252#comment-57537 What is impact of diluent and insufficient column equilibration on retention time of analyte.

]]> By: Dr. Deepak Bhanot https://lab-training.com/prevention-of-retention-time-drifts-in-hplc-analysis/#comment-1775 Thu, 09 Jan 2014 10:11:12 +0000 https://lab-training.com/?p=15252#comment-1775 In reply to Abhisek Ghosh.

Thanks Abhishek
Good to note that the article was useful.Special care should be taken in Reverse phase separations with regard to pH. It should be maintained between 2 and 8 to get long column life.

]]> By: Abhisek Ghosh https://lab-training.com/prevention-of-retention-time-drifts-in-hplc-analysis/#comment-1774 Thu, 09 Jan 2014 09:09:38 +0000 https://lab-training.com/?p=15252#comment-1774 Sir, thank you for the article.
It was informative.
” If lower than pH 2 media is used the stationary phase can get stripped of the organic functional groups”-
this particular information was new for me.

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